Journal: Drug Invention Today

Article Id: JPRS-MSNS-00003404
Title: Assessment of the outer membrane protein profile of Vibrio parahaemolyticus using the sodium dodecyl sulfate‑polyacrylamide gel electrophoresis method
Category: Microbiological Study (on Natural/ Synthetic drug)
Section: Research Article
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    Aims: The bacterium Vibrio parahaemolyticus causes gastroenteritis in humans after the consumption of raw seafood. Therefore, a rapid detection system that can identify the presence of V. parahaemolyticus accurately is necessary. One potential detection system under development is based on antibodies. This study aimed to map the outer membrane protein profile of V. parahaemolyticus. Methods and Results: These outer membrane proteins (OMP) may be antigenic and can be used to produce antibodies. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method was used to map the outer membrane protein profile of V. parahaemolyticus. The results of the analysis using SDS-PAGE to assess the OMP of V. parahaemolyticus show that the molecular weight (MW) of the major polypeptide band was in the range of 10–100 kDa. Overall, there were 31 bands observed, of which 11 were major bands with MW of 72, 67, 58, 44, 42, 40, 38, 37, and 10 kDa. Conclusion, Significance, and Impact of Study: The identified proteins have antigenic properties and can be used to produce antibodies for a rapid detection system (rapid test) to detect the presence of V. parahaemolyticus.

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    Author(s) Name:

    Mery Pattipeilohy1,2 *, Moh. Amin3 , Umie Lestari 3 , Endang Suarsini 3

    Affiliation(s) Name:

    1 Pattimura University lecturer, Indonesia, 2 Biology Education Programe, Faculty of Teacher Training and Education, Pattimura University, Indonesia, 3 Depaetment of Biology, Faculty of Mathematic and Natural Science, State University of Malang, Indonesia

    *Corresponding author: Mery Pettipeilohy, Biology Education Programe, Faculty of Teacher Training and Education, Pattimura University, Indonesia.

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    Author:

    Mery Pattipeilohy1,2 *, Moh. Amin3 , Umie Lestari 3 , Endang Suarsini 3

    Title:Assessment of the outer membrane protein profile of Vibrio parahaemolyticus using the sodium dodecyl sulfate‑polyacrylamide gel electrophoresis method
    Journal:Drug Invention Today
    Vol(issue):12 (April)
    Year:2019
    Page No: (684-689)
  • Experimental Methods Keywords

    Methodology:outer membrane protein profile , gel electrophoresis method
    Research Materials:Antibody

Keywords

Antibody Odium dodecyl sulfate-polyacrylamide gel electrophoresis Outer membrane protein profile Vibrio parahaemolyticus

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