Journal: Drug Invention Today
Title: In vitro cytotoxic activity of Asparagus racemosus on ovarian carcinoma cell lines (SKOV-3) by 3-(4, 5dimethythiazol2-yl) -2, 5-diphenyl tetrazolium bromide assay
Category: Pharmaceutics
Section: Research Article
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Abstract
Introduction: Natural products have been used since ancient times for the treatment of many diseases. Biodiversity and traditional medical knowledge had provided useful lead compounds for cancer chemotherapy. In the Indian medicine system, Asparagus racemosus, commonly called Shatavari has been used as a nutritive tonic, general female reproductive tonic, fertility tonic, treatment for sexual debility, and as an aphrodisiac. Aim: The aim of the present study is to study the in vitro cytotoxic effect of A. racemosus extracts on SKOV3 cells line (HTB77) using 3-(4, 5dimethythiazol2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Materials and Methods: SKOV3 cells line (HTB77) commercially purchased from the American Type Culture Collection were cultured in McCoy’s 5A modified medium supplemented with fetal bovine serum (heat inactivated, 10%) and penicillin-streptomycin (1%) at 37°C in 5% CO. MTT colorimetric assay that measures the reduction of yellow MTT by mitochondrial succinate dehydrogenase is used to check the viability of the cells. Result: It was observed that the extract showed promising anticancer activity toward the selected cancer cell line on dose-dependent manner. Conclusion: A. racemosus contains a rich source of steroidal saponins which are important secondary metabolites with cytotoxic activity. Furthermore, research should be directed toward the isolation of bioactive compounds from these plants.
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About the authors and Affiliations
Author(s) Name: S. Kalai Vani 1 *, Girija Sivakumar2 , R. V. Geetha3 , V. Vishnu Priya4
Affiliation(s) Name: 1 Department of Anatomy, Priyadarshini Dental College, Thiruvallur, Tamil Nadu, India,
2 Department of Anatomy, Karpaga Vinayaga Institute of Medical Sciences, Kanchipuram, Madurantagam, Tamil Nadu, India,
3 Department of Microbiology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India,
4 Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India
*Corresponding author: S. Kalai vani, Department of Anatomy, Priyadarshini Dental College, Thiruvallur, Tamil Nadu, India
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Author: S. Kalai Vani 1 *, Girija Sivakumar2 , R. V. Geetha3 , V. Vishnu Priya4
Title: In vitro cytotoxic activity of Asparagus racemosus on ovarian carcinoma cell lines (SKOV-3) by 3-(4, 5dimethythiazol2-yl) -2, 5-diphenyl tetrazolium bromide assay Journal: Drug Invention Today Vol(issue): 12 (June) Year: 2019 Page No: (1162-1165) -
Experimental Methods Keywords
Methodology: 3-(4, 5dimethythiazol2-yl)-2, 5-diphenyl tetrazolium bromide assay, Cytotoxic, SKOV3 cells line Research Materials: Asparagus racemosus
Keywords
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