Journal: Drug Invention Today

Article Id: JPRS-P col-00004082
Title: Antidiabetic activity of silver nanoparticles synthesized using neem and Aloe vera plant formulation
Category: Pharmaceutics
Section: Research Article
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    Introduction: Diabetes mellitus (DM) is a serious and chronic disease that can occur either when the pancreas fails to produce enough insulin (Type 1 DM/insulin-dependent DM) or when the body fails to use the insulin, it produces effectively (Type 2 DM/non-insulin-dependent DM). Since the prevalence of DM has only been exponentially increasing, the need for finding alternative forms of medication and treatment is of immense importance. Here, we have aimed to analyze the possible antidiabetic potential of silver nanoparticles (Ag-NPs) synthesized using neem and Aloe vera through an alpha-amylase assay. Materials and Methods: Leaves of Azadirachta indica and A. vera were made into a filtered herbal formulation. A metallic solution of silver nitrate was added to it and the conjunction was kept in a magnetic stirrer/orbital shaker for the synthesis of its nanoparticles. After centrifugation, the resultant pellet was powdered. The antidiabetic potential was studied through the inhibition of alpha-amylase. Ag-NP concentrations of 50, 100, and 150 μL were preincubated with 100 μL of alpha-amylase solution (1 U/mL) at room temperature for 30 min. About 100 μL of starch solution (1% w/v) was then added to it and the mixture was incubated at room temperature for 10 min. About 100 μL of 96 mM 3,5-dinitrosalicylic acid reagent was added to it to stop the reaction and the solution was heated in a water bath. Control was maintained where equal quantities of the enzyme and extract were replaced by a sodium phosphate buffer maintained at a pH of 6.9. The reading was measured at 540 nm. The experiment was performed thrice. Acarbose was used as a positive control and the percentage of inhibition was calculated. Results: A color change was observed after the synthesis of Ag-NPs. The prepared particles were then characterized by a peak seen at 425 nm in ultraviolet-visible spectroscopy. The results of the assay showed that 150 µg of our Ag-NPs exhibited an alpha-amylase inhibition which was close to that of acarbose with a variation of only 2.67%. Conclusion: Ag-NPs synthesized using neem and A. vera have an evident antidiabetic potential. They show a considerably good amount of alpha-amylase inhibition with respect to acarbose. They are also easy to synthesize, eco-friendly, and inexpensive. Thus, Ag-NPs could be used as a possible alternative for conventional antidiabetic drugs.

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    Author(s) Name:

    K. Sathvika, S. Rajeshkumar*, T. Lakshmi, Anitha Roy

    Affiliation(s) Name:

    Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: Dr. S. Rajeshkumar, Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600 077, Tamil Nadu, India

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    Author:

    K. Sathvika, S. Rajeshkumar*, T. Lakshmi, Anitha Roy

    Title:Antidiabetic activity of silver nanoparticles synthesized using neem and Aloe vera plant formulation
    Journal:Drug Invention Today
    Vol(issue):12 (September )
    Year:2019
    Page No: (2026-2029)
  • Experimental Methods Keywords

    Methodology:Antidiabetic activity,Silver nanoparticles
    Research Materials: Aloe vera

Keywords

Aloe vera Antidiabetic Diabetes Neem Silver nanoparticles

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