Journal: Drug Invention Today

Article Id: JPRS-P col-00004114
Title: Antibacterial efficacy of silver nanoparticles synthesized using herbal formulation against clinical pathogens
Category: Pharmaceutics
Section: Research Article
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    Introduction: Silver nanoparticles (AgNPs) have a wide range of application in a variety of fields such as medicine, food, cosmetics, and agriculture. They are used as antimicrobial and antifungal agents in agriculture and can potentially be used as targeted drugs for the human body. This antibacterial activity of AgNPs is being taken advantage of, in producing antibacterial textiles, coatings on medical devices, cosmetics, toothpastes, laundry detergents, soaps, home appliances, etc. The aim of this research is to study the antibacterial efficacy of AgNPs synthesized using herbal formulation against clinical pathogens. Materials and Methods: Herbal formulation was created using the leaves of Andrographis paniculata and Moringa oleifera. To this, silver nitrate solution was added and the solution was transferred to an orbital shaker for AgNP preparation. The powder of AgNPs was created. A fresh bacterial suspension of clinical pathogens such as Serratia, Aeromonas hydrophila, and Vibrio parahaemolyticus was cultured in Mueller-Hinton agar plates. Different concentrations of NPs (20, 40, and 60 µl) were incorporated into the wells and the plates were incubated at 37°C for 24 h. The antibiotics were used as positive control. Zone of inhibition was recorded in each plate. Proper documentation methods were used. The bioreduction of pure AgNO3 was characterized by ultraviolet (UV)-visible spectroscopy. Results: Color change was seen after the preparation of AgNPs. The peak in UV-visible spectroscopy was seen to be at 390 nm. The zone of inhibition was seen to increase with an increase in the concentration of AgNPs in the agar wells. The zone was smallest in A. hydrophila sp. culture, whereas it was seen to be the largest in Serratia sp. culture. Conclusion: We can conclude by saying that herbal synthesis of AgNPs is highly eco-friendly and should be practiced more than chemical synthesis of the same. The antibacterial activity of AgNPs was comparably effective to the control used and in the future, they can be administered as antibiotics in the era of multidrug resistance.

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    Author(s) Name:

    Ankita Komal Labh, S. Rajeshkumar*, T. Lakshmi, Anitha Roy

    Affiliation(s) Name:

    Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: Dr. S. Rajeshkumar, Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, 162, Poonamallee High Road, Chennai - 600 077, Tamil Nadu, India

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    Author:

    Ankita Komal Labh, S. Rajeshkumar*, T. Lakshmi, Anitha Roy

    Title:Antibacterial efficacy of silver nanoparticles synthesized using herbal formulation against clinical pathogens
    Journal:Drug Invention Today
    Vol(issue):11 (October )
    Year:2019
    Page No: (2362-2365)
  • Experimental Methods Keywords

    Methodology: Antibacterial activity
    Research Materials:Herbal synthesis, Silver nanoparticles

Keywords

Antibacterial Clinical pathogens Herbal synthesis Silver nanoparticles

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