Journal: Drug Invention Today

Article Id: JPRS-P col-00004123
Title: Purification and characterization of protease and lipase from Pseudomonas aeruginosa isolated from some wound and burn infection
Category: Pharmaceutics
Section: Research Article
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    Background: Pseudomonas aeruginosa is a type of bacteria that causes many diseases, especially hospital infections (nosocomial infections) because of its wide spread in the environment, especially the hospital environment. Methodology: Twenty isolates of P. aeruginosa were identified. Results: The isolates were 12 isolates from burn infections and 8 isolates from wound infections. The sensitivity of P. aeruginosa isolates was tested against (6) traditional antibiotics. P. aeruginosa was (100%) resistance to Ampicillin, 94.5% resistance to Cefixime, (80%) to Ceftazidime, (38.9%) to Tobramycin, 22.9% to Norfloxacillin and 3.4% to Ciprofloxacin . By observing hydrolysis zone around the colony of bacteria that grow on Peptone tween agar plate, we confirmed the lipolytic ability and produce lipase enzyme. Their abilities for production of protease enzyme was tested on skim milk agar by appeared clear zone round the colony. The optimum conditions of enzymes production were studied such as (PH, temperature) which show that 40°C , pH (8) and incubating to 48 hr. Conclusion: The enzyme Activity was decremented in these optimum conditions and The activity of the enzyme has increased when treated with Ca ions, which shows that Ca++ plays a role in the stimulation and stability of the enzyme, and did not inhibit the activity of the enzyme with the presence of reduced agents such as cysteine, while decrease the enzyme’s effectiveness of the factors EDT.

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    Author(s) Name:

    Yazi Abdullah Jassim, Shaemaa Muhi Hasson Al-Amery*

    Affiliation(s) Name:

    Department of Biology, College of Science, University of Babylon, Babylon Province, Iraq

    *Corresponding author: Shaemaa Muhi Hasson Al-Amery, Department of Biology, College of Science, University of Babylon, Babylon Province, Iraq.

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    Author:

    Yazi Abdullah Jassim, Shaemaa Muhi Hasson Al-Amery*

    Title:Purification and characterization of protease and lipase from Pseudomonas aeruginosa isolated from some wound and burn infection
    Journal:Drug Invention Today
    Vol(issue):11 (October )
    Year:2019
    Page No: (2400-2406)
  • Experimental Methods Keywords

    Methodology:Purification and characterization ,
    Research Materials:Proteolytic enzyme

Keywords

 Nosocomial infection P. aeruginoas Proteolytic enzyme

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