Journal: Drug Invention Today

Article Id: JPRS-P col-00004248
Title: Pro-inflammatory role of leptin in peripheral blood mononuclear cells
Category: Pharmaceutics
Section: Research Article
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    Background: Hyperleptinemia is a pathological condition that mediates activation of inflammatory cells and trigger events that promote atherosclerosis. In the present study, we investigated the signaling mechanisms (JAK-2/STAT-3 pathway) underlying the pro-inflammatory role of leptin in peripheral blood mononuclear cells (PBMCs). Materials and Methods: PBMCs were isolated from healthy volunteers and treated with leptin in the presence or absence of JAK-2 inhibitor, tyrphostin AG490. The mRNA and protein levels of TNF-α and inducible nitric oxide synthase (iNOS) were then analyzed by ELISA and reverse transcription-PCR, respectively. NO levels were measured using Griess reagent. Results: Leptin activated PBMCs and promoted the synthesis of TNF-α, iNOS, and NO. However, exposure of PBMCs to tyrphostin AG490 significantly reduced the protein levels of both TNF-α and iNOS and decreased the secretion of NO. Conclusion: Leptin augments inflammatory response in PBMCs through JAK-2/STAT-3 pathway.

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    Author(s) Name:

    D. Nalini 1 , P. Hema2 , J. Selvaraj 3 *

    Affiliation(s) Name:

    1 Central Research Laboratory, Meenakshi Ammal Dental College and Hospital, Chennai, Tamil Nadu, India,

    2 Department of Pharmacology, Coimbatore Medical College, Coimbatore, Tamil Nadu, India,

    3 Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: Dr. J. Selvaraj, Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, 162, Poonamallee High Road, Velappanchavadi, Chennai - 600 077, Tamil Nadu, India.

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    Author:

    D. Nalini 1 , P. Hema2 , J. Selvaraj 3 *

    Title:Pro-inflammatory role of leptin in peripheral blood mononuclear cells
    Journal:Drug Invention Today
    Vol(issue):12 (October)
    Year:2019
    Page No: (2381-2384)
  • Experimental Methods Keywords

    Methodology: iNOS, Leptin, NO, Peripheral blood mononuclear cells, TNF-α
    Research Materials:Leptin

Keywords

iNOS Leptin NO Peripheral blood mononuclear cells TNF-α

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