Journal: Drug Invention Today

Article Id: JPRS-DA-00004411
Title: Reversed-phase high-performance liquid chromatography method development and validation of ilaprazole and domperidone in bulk and pharmaceutical dosage forms
Category: Drug Analysis
Section: Research Article
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    Objective: A simple, accurate, and precise reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the simultaneous estimation of the ilaprazole (ILA) and domperidone (DOM) in bulk and pharmaceutical dosage forms. Materials and Methods: Chromatogram was run through Zodiac Sil RP C18 (4.5 mm × 100 mm 3.0 µm) with a mobile phase consisting of 70:30 methanol:phosphate buffer at a flow rate of 1 ml/min. Detection was carried out at 240 nm. The developed method was validated in terms of accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and solution stability. Results: The retention time of ILA and DOM was found to be 2.170 min and 7.280 min, and percentage relative standard deviation of ILA and DOM was found to be 0.52% and 0.83%, respectively. The described method shows excellent linearity over a range of 16–80 ppm of ILA and 25–150 ppm of DOM. The correlation coefficient for ILA and DOM was 0.999 and 0.999, respectively. The LOD was found to be 2.17 and 0.0372 and the LOQ values were 6.60 and 0.112, respectively. Percentage assay was obtained as 99.1% and 98.2% for ILA and DOM, respectively. Conclusion: The results of this study showed that the proposed RP-HPLC method is simple, rapid, precise, and accurate which is useful for the routine determination of ILA and DOM bulk drug and in its pharmaceutical dosage form.

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  • About the authors and Affiliations

    Author(s) Name:

    Sunkara Namratha1 , A. Vijayalakshmi 2 *

    Affiliation(s) Name:

    1 Department of Pharmaceutical Analysis, Bharat Institute of Technology, Ibrahimpatnam, Telangana, India,

    2 Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Chennai, Tamil Nadu, India

    *Corresponding author: A. Vijayalakshmi, Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai - 600 117, Tamil Nadu, India.

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    Author:

    Sunkara Namratha1 , A. Vijayalakshmi 2 *

    Title:Reversed-phase high-performance liquid chromatography method development and validation of ilaprazole and domperidone in bulk and pharmaceutical dosage forms
    Journal:Drug Invention Today
    Vol(issue):11 (November )
    Year:2019
    Page No: (3052-3056)
  • Experimental Methods Keywords

    Methodology:Reversed-phase high-performance liquid chromatography, Estimation, Simultaneous, Validation
    Research Materials: Ilaprazole and domperidone

Keywords

Estimation Ilaprazole and domperidone Reversed-phase high-performance liquid chromatography Simultaneous Validation

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