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Abstract

Objective: A simple, accurate, and precise reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the simultaneous estimation of the ilaprazole (ILA) and domperidone (DOM) in bulk and pharmaceutical dosage forms. Materials and Methods: Chromatogram was run through Zodiac Sil RP C18 (4.5 mm × 100 mm 3.0 µm) with a mobile phase consisting of 70:30 methanol:phosphate buffer at a flow rate of 1 ml/min. Detection was carried out at 240 nm. The developed method was validated in terms of accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and solution stability. Results: The retention time of ILA and DOM was found to be 2.170 min and 7.280 min, and percentage relative standard deviation of ILA and DOM was found to be 0.52% and 0.83%, respectively. The described method shows excellent linearity over a range of 16–80 ppm of ILA and 25–150 ppm of DOM. The correlation coefficient for ILA and DOM was 0.999 and 0.999, respectively. The LOD was found to be 2.17 and 0.0372 and the LOQ values were 6.60 and 0.112, respectively. Percentage assay was obtained as 99.1% and 98.2% for ILA and DOM, respectively. Conclusion: The results of this study showed that the proposed RP-HPLC method is simple, rapid, precise, and accurate which is useful for the routine determination of ILA and DOM bulk drug and in its pharmaceutical dosage form.

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	JPR Solutions

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