Journal: Drug Invention Today

Article Id: JPRS-MSNS-00004416
Title: Efficiency of antifungal agents with culture filtrates of two basidiomycetes fungi on growth and on citrate and isocitrate genes of Trichophyton rubrum
Category: Microbiological Study (on Natural/ Synthetic drug)
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    Background: Trichophyton rubrum is the most common dermatophyte in the world; it colonizes the upper layers of dead skin, causing athlete’s foot, fungal infection of nail, jock itch, and ringworm worldwide. Aim: The experiment aimed to evaluate the combinatorial efficacy of the basidiomycetes species (Marasmius palmivorus and Pleurotus ostreatus) filtrates with terbinafine on the growth and on citrate and isocitrate genes of the clinical isolate T. rubrum. Methodology: The combined efficiency of terbinafine with filtrates of both the fungal species on citrate and isocitrate genes of this pathogen was determined using real-time polymerase chain reaction (RT-PCR). Results: The results demonstrated the inhibitory effect of different concentrations of P. ostreatus and M. palmivorus filtrates (3, 6, 9, and 12%) on the growth of T. rubrum. The maximum inhibition rate to the growth of this pathogen reached 50.7% and 28.16% with P. ostreatus and M. palmivorus filtrates, respectively, at 12% concentration. On the other hand, the minimum inhibitory rate was reduced to 35.7% and 19% by P. ostreatus and M. palmivorus filtrates, respectively, at 3% concentration, compared with the maximum (50.51%) and minimum (35.29%) inhibitory rate by terbinafine at 0.006 µg/ml concentration. The -RT-PCR experiments revealed that basidiomycetes filtrates markedly affected the gene expression by downregulating the isocitrate and citrate genes in T. rubrum. In addition, the combination between terbinafine and P. ostreatus and M. palmivorus filtrates in this study showed a similar tendency by downregulation in isocitrate synthase and citrate synthase compared to the housekeeping gene (ß-tubulin genes in strain of T. rubrum). Conclusions: This innovative study focuses on the combinatorial application of culture filtrates with a distinguished antifungal agent, terbinafine, for the treatment of severe infections associated, with T. rubrum-exhibiting downregulation of citrate and isocitrate genes as antifungal targets. Moreover, the filtrates of both fungi might be a potential source of new antifungal agents and could serve as an alternative drug against T. rubrum.

  • Abstract Audio

    No Audio file found

  • About the authors and Affiliations

    Author(s) Name:

    Marwa H. Abdullah1 , Jawad K. A. Al-Janabi 2 *

    Affiliation(s) Name:

    1 Department of Biology, College of Science, University of Babylon, Hillah, Babil, Iraq,

    2 Department of Pathological Analysis Techniques, Al-Mustaqbal University College, Hillah, Babil, Iraq

    *Corresponding author: Jawad K. A. Al-Janabi, Department of Pathological Analysis Techniques, Al-Mustaqbal University College, Hillah, Babil, Iraq.

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    0 View More
  • How to Cite my Article.

    Author:

    Marwa H. Abdullah1 , Jawad K. A. Al-Janabi 2 *

    Title:Efficiency of antifungal agents with culture filtrates of two basidiomycetes fungi on growth and on citrate and isocitrate genes of Trichophyton rubrum
    Journal:Drug Invention Today
    Vol(issue):11 (November )
    Year:2019
    Page No: (3085-3096)
  • Experimental Methods Keywords

    Methodology:real-time polymerase chain reaction (RT-PCR)
    Research Materials:Antivirulence agents

Keywords

Antivirulence agents Citrate genes Isocitrate Marasmius palmivorus Pleurotus ostreatus Trichophyton rubrum

Our Services

Most Downloaded List