Journal: Drug Invention Today
Title: Alkaline protease of new bacterial species MTCC 5468 for chemical free dehairing of skin in leather making
Category: Biotechnology & Related Pharmaceutical Technology
Section: Research Article
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Abstract
Aim: Alkaline protease secreting bacterium was identified by 16S rRNA sequence analysis and named as Bacillus crolab MTCC 5468. Materials and Methods: The enzyme created zone of hydrolysis in zymography and agar-casein plate and therefore used for production of protease. Optimum conditions for enzyme production and activity were standardized. Results: The crude enzyme showed maximum activity at 40°C, pH 9.0. The enzyme thus produced showed 855 U/ml activity. It was more stable in the presence of Li + followed by NH4 + and Ca 2 + salts, and affected by Hg2 +. The enzyme depilated was able to goat hair effectively without chemical aid. Thus enzymatically dehaired skin was characterized by histology and SEM. Also the enzyme successfully removed blood stain on white cloth. Conclusion: The alkaline protease of this newly identified organism can be used in leather making and laundry industries for pollution abatement.
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About the authors and Affiliations
Author(s) Name: M. Fernandus Durai, K. A. Ridhanyalatha, P. Roshan Vaid, C. Rose*
Affiliation(s) Name: Department of Biotechnology, Central Leather Research Institute, Chennai, Tamil Nadu, India
*Corresponding author: Dr. C. Rose, Department of Biotechnology, Central Leather Research Institute, Chennai, Tamil Nadu, India
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Author: M. Fernandus Durai, K. A. Ridhanyalatha, P. Roshan Vaid, C. Rose*
Title: Alkaline protease of new bacterial species MTCC 5468 for chemical free dehairing of skin in leather making Journal: Drug Invention Today Vol(issue): 12 (November ) Year: 2019 Page No: (2498-2504) -
Experimental Methods Keywords
Methodology: Eco-friendly dehairing, Histology, Scanning electron micrograph Research Materials: new bacterial species MTCC 5468
Keywords
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