Journal: Drug Invention Today

Article Id: JPRS-Pcol-00004439
Title: Antiendometrial cancer activity of aqueous extract Hypnea valentiae with its protein isolation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique
Category: Pharmacology
Section: Research Article
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    Objective: The purpose of the present research work was to investigate the antiendometrioid activity of red seaweed Hypnea valentiae with its protein isolation technique. Materials and Methods: The marine seaweed having potent source of protein content. Selected extracted H. valentiae screened by MTT assay by using HEC1A, which is endometrial cancer cell (ECC) line compared with the normal endometrial cell line. Electrophoresis is widely used to separate and characterize proteins by applying electric current and is rapid and relatively sensitive requiring only microweight of proteins. Electrophoresis in polyacrylamide gel is carried out in buffer gel (non-denaturing) as well as sodium dodecyl sulfate (SDS)-containing denaturing gel. Polyacrylamide gel is formed with linking agent (bisacrylamide) in the presence of a catalyst (persulfate ion) and chain inhibition (TEMED). The relative proportion of acrylamide to the bisacrylamide determines the porosity of gel. At low percentage, gel (with large pore size) is used to separate concentrate ammonium persulfate and TEMED, which increases the amount of polymerized ion of the gel. SDS is anionic detergent which binds strongly and denatures protein. The number of SDS molecule bounds to a polypeptide chain approximately half the number of amino acid residue in that chain. The protein SDS complex carries net negative charges and hence moves toward the anode and separation is based on the size of the protein. Results: Aqueous extract of H. valentiae was found with shown significant activity at 15.32 µg/ml at HEC1A cell line and 12.58 µg/ml at ECC line, in case of normal endometrial cell line, cell necrosis started at 140.52 µg/ml. Hence, the potent seaweed having the valuable cytotoxic effect. Conclusion: Seaweeds had a potent carcinogenetic activity; in this, the antiendometriodial activity of H. valentiae (red seaweed) was proved. Nowadays, gynecological dysfunction of women should be a greater challenges, the seaweeds are useful remedies.

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    Author(s) Name:

    S. Jayakumari*, S. Dhanalakshmi

    Affiliation(s) Name:

    Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Chennai, Tamil Nadu, India

    *Corresponding author: S. Jayakumari, Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai - 600 077, Tamil Nadu, India.

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    Author:

    S. Jayakumari*, S. Dhanalakshmi

    Title:Antiendometrial cancer activity of aqueous extract Hypnea valentiae with its protein isolation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique
    Journal:Drug Invention Today
    Vol(issue):12 (November )
    Year:2019
    Page No: (2562-2565)
  • Experimental Methods Keywords

    Methodology:Antiendometrial cancer activity
    Research Materials:Hypnea valentiae

Keywords

Hypnea valentiae Protein isolation Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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