Journal: Drug Invention Today

Article Id: JPRS-Pcol-00004446
Title: Low-dose corticosteroid and Vitamin C effects on endothelial cell glycocalyx expression in sepsis model human umbilical vein endothelial cells culture
Category: Pharmacology
Section: Research Article
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    Objective: The main factor of sepsis condition is not a bacterial infection, but the inflammatory reaction to the infection and research in sepsis condition has progressed to glycocalyx layer or endothelial surface layer. Corticosteroids and antioxidants treatment are one of the strategies to protect glycocalyx and to prevent damage or accelerate the recovery. Materials and Methods: The objective of this study is to investigate the low dose of corticosteroid and Vitamin C effects on endothelial cell glycocalyx expression using sepsis conditioned human umbilical vein endothelial cells (HUVECs) culture. HUVEC culture used umbilicus obtained from caesarian section childbirth, and treated with lipopolysaccharide-conditioned monocyte medium exposure and steroid and Vitamin C treatment. Immunofluorescence staining was then performed to the samples. Results: The result shows that corticosteroid and Vitamin C have been proven to have a protective effect on glycocalyx layer under sepsis condition by suppressing inflammatory response to infection and antioxidant effect. Conclusion: 20 μM steroid, 75 μM Vitamin C, and 10 μM steroid combined with 50 μM Vitamin C gave the most significant glycocalyx thickness layer after 8 h of treatment, even though combined treatment does not as effective as single treatment.

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    Author(s) Name:

    Purwoko Purwoko*

    Affiliation(s) Name:

    Department of Anesthesiology and Intensive Therapy, Sebelas Maret University, Dr. Moewardi General Hospital, Surakarta, Central Java 57126, Indonesia

    *Corresponding author: Purwoko Purwoko, Department of Anesthesiology and Intensive Therapy, Sebelas Maret University, Dr. Moewardi General Hospital, Jalan Kolonel Sutarto No. 132 Surakarta, Central Java 57126, Indonesia

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    Author:

    Purwoko Purwoko*

    Title:Low-dose corticosteroid and Vitamin C effects on endothelial cell glycocalyx expression in sepsis model human umbilical vein endothelial cells culture
    Journal:Drug Invention Today
    Vol(issue):12 (November )
    Year:2019
    Page No: (2598-2604)
  • Experimental Methods Keywords

    Methodology:Immunofluorescence staining, Human umbilical vein endothelial cells culture, Inflammatory effect, Sepsis
    Research Materials: Vitamin C, Corticosteroid,

Keywords

Corticosteroid Glycocalyx Human umbilical vein endothelial cells culture Inflammatory effect Sepsis Vitamin C

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