Journal: Drug Invention Today

Article Id: JPRS-Pcol-00004604
Title: In vitro assessment of cytotoxicity and antiproliferation activities of the ethyl acetate fraction from Solanum torvum fruit powder toward T47D breast cancer
Category: Pharmacology
Section: Research Article
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    Background: The aim of this study was to analyze the antioxidant content of the ethyl acetate fraction of Solanum torvum fruit against cytotoxic effects, selectivity index, cell cycle, and apoptosis of T47D breast cancer cells. Materials and Methods: The response surface methodology-Box-Behnken design was used as the experimental design. In addition, liquid chromatography mass spectrometry (LCMS), 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide assay, and FACSCalibur flow cytometry were used in this research. Moreover, half-maximal inhibitory concentration (IC50 ) of the cytotoxic treatments was statistically evaluated by Microsoft Excel. Results: The results showed that antioxidant activity 2,2-diphenyl 1-picrylhydrazyl of S. torvum fruit was 87.54%, the polyphenol content was 61.61 mg gallic acid equivalents/g, the tannin content was 1.06 mg tannic acid equivalents/g, and the flavonoid content was 1.72 mg QAE/g. Meanwhile, the G0-G1 rate was 46.91%, the S-phase rate was 20.91%, and the G2-M rate was 31.25%. S. torvum fruit can increase initial apoptosis and reduce the damaging effect of cell membranes (R2) by 25.19%. In region 3 (R3), late apoptosis increased inducing cell membrane damage by 48.06%, and the necrosis rate was 13.26%. The LCMS chemical compound showed that S. torvum dried fruit contains chlorogenic acid, 4-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3-O-feruloylquinic acid, 5-O-feruloylquinic acid, 3-O-feruloylquinic acid, 3,4-O dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 3-O-feruloylquinic acid, 5-O-feruloylquinic acid, and putative dicaffeoylquinic lactone. The results showed that polyphenols, tannins, flavonoids, chlorogenic acids, and their derivatives in the ethyl acetate fraction of S. torvum dry fruit have cytotoxic activity with IC50 , approximately 39.17 µg/ml, and have an antiproliferation activity. Conclusion: These results suggest that S. torvum fruits might be potent as chemopreventive agents against T47D breast cancer cells.

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    Author(s) Name:

    Nunuk Helilusiatiningsih1,2 , Yunianta Yunianta2 , Harijono Harijono2 , Simon Bambang Widjanarko2 *

    Affiliation(s) Name:

    1 Department of Agrotechnology, Faculty of Agriculture, Kediri Islamic University, Kediri, East Java, Indonesia,

    2 Department of Agricultural Industrial Technology, Brawijaya University, Malang, Indonesia

    *Corresponding author: Simon Bambang Widjanarko, Department of Agricultural Industrial Technology, Brawijaya University, Malang, Indonesia.

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    Author:

    Nunuk Helilusiatiningsih1,2 , Yunianta Yunianta2 , Harijono Harijono2 , Simon Bambang Widjanarko2 *

    Title:In vitro assessment of cytotoxicity and antiproliferation activities of the ethyl acetate fraction from Solanum torvum fruit powder toward T47D breast cancer
    Journal:Drug Invention Today
    Vol(issue):11 (December )
    Year:2019
    Page No: (3191-3201)
  • Experimental Methods Keywords

    Methodology:Apoptosis, Cell cycle, Cytotoxicity, T47D cancer cell
    Research Materials:Solanum torvum fruit

Keywords

Apoptosis Cell cycle Cytotoxicity Solanum torvum fruit T47D cancer cell

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