Journal: Drug Invention Today

Article Id: JPRS-Pcol-00004616
Title: Production and purification of killer toxins associated with new record of four Debaryomyces species in Iraq
Category: Pharmacology
Section: Research Article
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    Background: Ecological killer yeasts have been less studied compared to laboratory and domesticated killer yeasts. Killer yeasts secrete so-called killer toxins that inhibit close sensitive yeasts. These toxins assumed to be tools for studying ability as antifungal and antimicrobial agents. Materials and Methods: Soil samples were collected from different regions in Basrah Province using CHROMagar Candida, VITEK, and polymerase chain reaction (PCR) for morphology, biochemical, and genetic identification beside of determined gene expression for strains purification and identification killer toxin for the best strains which produced high production of killer toxin in fermentation medium. Results: The results of this study showed that a record of four ascomycetes species belongs to genus Debaryomyces for the 1st time in Iraq. Biochemical and VITEK tests were used for identification as well as a molecular study represented by PCR and DNA sequencing of internal transcribed spacer (ITS1)-ITS2 region rDNA gene was conducted for more accurate discrimination between morphological alike species. Total RNA samples were extracted from yeast colonies and the expression of L-A, M1, and M2 genes was measured by real-time PCR. The highest gene expression was for Debaryomyces vindobonensis (DV), while the lowest was for Debaryomyces fabryi. The killer toxins were purified using Sephadex G75 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis; then, killer assay was performed against clinical isolates showing that the zone of inhibition was less against bacteria. Conclusion: D. vindobonensis had the highest gene expression for killer toxins K1, K2, and K28 as well as had a high antimicrobial activity.

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    Author(s) Name:

    Najwa Mohammed Jameel Ali Abu-Mejdad*

    Affiliation(s) Name:

    Department of Biology, College of Science, Basra University, Basra, Iraq

    *Corresponding author: Najwa Mohammed Jameel Ali Abu-Mejdad, Department of Biology, College of Science, Basra University, Basra, Iraq

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    Author:

    Najwa Mohammed Jameel Ali Abu-Mejdad*

    Title:Production and purification of killer toxins associated with new record of four Debaryomyces species in Iraq
    Journal:Drug Invention Today
    Vol(issue):12 (December )
    Year:2019
    Page No: (2857-2864)
  • Experimental Methods Keywords

    Methodology:Antimicrobial activity
    Research Materials:Debaryomyces

Keywords

Antimicrobial activity Debaryomyces Killer toxin genes Real-time polymerase chain reaction

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