Journal: Drug Invention Today

Article Id: JPRS-P'Col-00004817
Title: Beta-sitosterol improves the antioxidant enzyme activity in liver: High-fat diet-induced type 2 diabetic experimental study
Category: Pharmacology
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    Background: Changes in lifestyle, such as high-calorie diet intake and lack of exercise, have increased the global prevalence of obesity and diabetes. The major pathophysiological event contributing to the development of type 2 diabetes mellitus is the target tissues to insulin action. β-sitosterol (24-ethyl cholestene-3-ol), a naturally occurring plant sterol, ubiquitously found in many plants such as wheat germ, rice bran, flax seeds, peanuts, soybean, and pumpkin seeds. It has been reported to elicit multitude of bioactivities including antihyperlipidemic and antihyperglycemic activity. Aim: The study was aimed at assessing the effect of β-sitosterol on antioxidant enzymes in high-fat diet-induced type-2 diabetic rats. Experimental Design: Adult male Wistar rats were used and divided as Group I: Control; Group II: Type-2 diabetic rats; Group III: Type-2 diabetic rats treated with β-sitosterol (20 mg/kg body weight [b.wt]/day, orally for 30 days); and Group IV: Type-2 diabetic rats treated with metformin (50 mg/kg b.wt/day, orally for 30 days). After 30 days of treatment, animals were anesthetized; liver tissues were dissected out and used for the assessment of antioxidant enzymes (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPx], glutathione-S-transferase [GST], and glutathione reductase [GR]). Results: SOD, CAT, GPx, GST, and GR enzyme activity was found to be significantly reduced (P < 0.05) in type-2 diabetic rats when compared with control animals. Oral administration of the plant sterol could effectively improve the activity of these enzymes (P < 0.05) to that of the control and standard drug metformin. Conclusion: The present study shows that β-sitosterol exhibits antidiabetic activity by facilitating antioxidant enzymes in the liver.

  • Abstract Audio

    No Audio file found

  • About the authors and Affiliations

    Author(s) Name:

    R. Saravanan1,2, R. Ponnulakshmi3, K. Revathy3, D. Anandhi1, G. Ramajayam4, Selvaraj Jayaraman5*

    Affiliation(s) Name:

    1Central Research Laboratory, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu, India.

    2Department of Biochemistry, Karpaga Vinayaga Institute of Dental Science, Chinnakolampakkam, Chengalpat District-603308, Tamil Nadu, India .

    3Central Research Laboratory, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu, India.

    4Multi Disciplinary Research Unit, Madurai Medical College, Madurai, Tamil Nadu, India.

    5Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.

    *Corresponding author: Dr. Selvaraj Jayaraman, Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600 077, Tamil Nadu, India.

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    0 View More
  • How to Cite my Article.

    Author:

    R. Saravanan1,2, R. Ponnulakshmi3, K. Revathy3, D. Anandhi1, G. Ramajayam4, Selvaraj Jayaraman5*

    Title:Beta-sitosterol improves the antioxidant enzyme activity in liver: High-fat diet-induced type 2 diabetic experimental study
    Journal:Drug Invention Today
    Vol(issue):13 (February )
    Year:2020
    Page No: (334-338)
  • Experimental Methods Keywords

    Methodology:Antioxidant enzymes, Type-2 diabetes
    Research Materials:Beta-sitosterol, High-fat diet

Keywords

Antioxidant enzymes Beta-sitosterol High-fat diet Type-2 diabetes

Our Services

Most Downloaded List