Journal: Journal of Pharmacy Research
Title: Phytochemical investigations and in vitro evaluation of Nyctanthes arbor-tristis leaf extracts for antioxidant property
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
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Abstract
The decoction of the leaves of Nyctanthes arbor-tristis Linn. widely used in Ayurvedic system of medicine for the treatment of sciatica, arthritis, fevers and various painful conditions.The leaf was studied for Pharmacognostic evaluations, including examination of morphological and microscopic characters, determination of leaf constants, ash values and extractive values. The dried leaves of Nyctanthes arbortristis were subjected to prilimiary phytochemical screening by extracting exhaustively the crude drug with alcohol in a Soxhlet extractor. The extract was concentrated using a rotary flash evaporator, residue was dried in a dessicator over sodium sulfite to give a semisolid mass. This alcoholic extract was further fractionated in to Pet ether, ethyl acetate, butanol and aqueous fractions. Preliminary Phytochemical investigations showed the presence of Flavonoids, Carbohydrates, Alkaloids, Phytosterols, Phenolic Compounds and Glycosides. Free radical scavenging potential of the different extracts of leaves of Nyctanthes arbor-tristis, was evaluated in vitro by using diphenyl-picrylhydrazyl( DPPH) assay. In this method the antioxidants present in the plant extracts reacted with DPPH, which is a stable free radical and converted it to 1,1-diphenyl-1,2-picryl, hydrazine which is measured at 517 nm. The scavenging effect of plant extracts and standard (ascorbic acid and BHT) on the DPPH radical decreases in the following order: ascorbic acid > Butanol > Ethyl acetate >BHT > Pet ether and ascorbic acid was found to be 93.88% at concentration of 10 mg, BHT, Butanol, Ethyl acetate and Pet ether was found to be 97.42%, 95.22% 84.63% and 82.04% at concentration of 100 mg respectively. In the present study, different extracts of Nyctanthes arbor-tristis leaves showed concentration dependent free radical scavenging activity.
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About the authors and Affiliations
Author(s) Name: Kusum S. Akki*, G Krishnamurthy1 and H. S. Bhoja naik2
Affiliation(s) Name: *Department of Pharmacognosy& Phytochemistry,K.L.E.S’s College of Pharmacy, Vidyanagar, Hubli Karnataka,India.
1Department of Chemistry, Sahyadri Science College, Shimoga, Karnataka, India.
2Department of Industrial chemistry, Kuvempu University, Jnana Sahyadri Shimoga ,Karnataka, India.
* For Correspondence:Kusum S. Akki
Received on:17-12-2008; Accepted on :22-02-2009-
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Author: Kusum S. Akki*, G Krishnamurthy1 and H. S. Bhoja naik2
Title: Phytochemical investigations and in vitro evaluation of Nyctanthes arbor-tristis leaf extracts for antioxidant property Journal: Journal of Pharmacy Research Vol(issue): 2 (April) Year: 2009 Page No: (752-755) -
Experimental Methods Keywords
Methodology: Phytochemical investigations and in vitro evaluation Research Materials: Nyctanthes arbor-tristis
Keywords
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