Journal: Drug Invention Today
Title: Molecular characterization and gene expression profiling of Trichophyton rubrum treated with a Marasmius palmivorus filtrate
Category: Pharmacology
Section: Research Article
- Abstract
- Audio Abstract
- Authors
- Pdf File
- Citation
- My Reference
- Methodology
-
Abstract
Background: Trichophyton rubrum is a common anthropophilic fungal species. It is the primary causative dermatophytosis agent in the world, infecting the skin, feet, and the area between the fingernails. Aim: The present study examines the molecular characteristics of T. rubrum isolates and the bioactivity of Marasmius palmivorus filtrate against this fungus. Materials and Methods: Clinical isolates were collected from patients with different varieties of tinea who attended the Al-Hussein Hospital (Dermatology and Venereal Disease Unit) in Kerbala Province from February to November 2018. Results: Four T. rubrum isolates were identified based on their morphological and molecular features and were registered in the International GenBank with the accession numbers: MK167434, MK167437, MK167442, and MK167443, revealing close relationship with National Center for Biotechnology Information-Blast T. rubrum (MH865940.1). The phylogenetic tree of internal transcribed spacer rRNA sequences showed that the T. rubrum isolates (numbers 1, 2, 5, and 6) were closely related to global isolates of the same fungus. The results showed that this fungus could produce protease enzymes. M. palmivorus filtrate (2%, 4%, 6%, and 8%) showed an inhibitory influence on T. rubrum growth through the distortion of fungal structures (presence of abnormal mycelium and cell lysis). Gene expression was detected by quantitative real-time polymerase chain reaction (PCR) using the specific primer β-tubulin (housekeeping gene), a serine protease, citrate synthase, and MEP4. The amplification accuracy of the gene product was observed. The data revealed high gene copy amplification in serine protease, citrate synthase, appeared the accumulation of product through the duration of the qPCR experiment, but on the contrary, it happened with MEP4, which indicates protein lysis. Conclusion: The results of this study will aid in the development of innovative and novel effective drug targets and antifungal agents using M. palmivorus.
-
Abstract Audio
No Audio file found
About the authors and Affiliations
Author(s) Name: Nadia N. H. Al-Masaoodi1, Jawad K. Abood Al-Janabi2*, Ban Taha Mohammed1
Affiliation(s) Name: 1 Department of Biology, Pure Science College, University of Kerbala, Iraq,
2 Department of Pathological Analysis Techniques, AL-Mustaqbal University College, Hilla, Iraq
*Corresponding author: Jawad K. Abood Al-Janabi, Department of Pathological Analysis Techniques, AL-Mustaqbal University College, Hilla, Iraq.
-
View Article File in pdf format.
Article File
-
View Article Citation Here.
0 View More -
How to Cite my Article.
Author: Nadia N. H. Al-Masaoodi1, Jawad K. Abood Al-Janabi2*, Ban Taha Mohammed1
Title: Molecular characterization and gene expression profiling of Trichophyton rubrum treated with a Marasmius palmivorus filtrate Journal: Drug Invention Today Vol(issue): 14 (March) Year: 2020 Page No: (109-119) -
Experimental Methods Keywords
Methodology: Molecular characterization and gene expression profiling Research Materials: Marasmius palmivorus filtrate
Keywords
Our Services
- Extraction of Betacyanin from Red Beet root (Beta vulgaris L.) and to evaluate its antioxidant potential antioxidant potential
- Analysis of Amino Acids By Paper Chromatography
- Formulation and evaluation of Aloe vera gels
- Sleep deprivation and cell phone usage among teenagers
- Awareness of selfie addiction among teenagers: A questionnaire-based study
- Effect of Kapalbhati Pranayama in the blood sugar level in diabetic patients
- Biodegradable plastic production from corn starch
- Citric acid production from cane molasses using submerged fermentation by Aspergillus niger ATCC9142
- Awareness of effects of sleep deprivation among college students
- Review On: Fast Dissolving Tablet
- Impurity profile- A review
- Cow Urine Concentrate : A potent agent with Antimicrobial and Anthelmintic activity
- Requirements of biopharmaceutics and clinical research for abbreviated new drug application submission – USA
- Antidiabetic activity of insulin plant (Costus igneus) leaf extract in diabetic rats
- Academic stress and its effect on medical students’ mental health status
- Trend of caffeine consumption among medical students and its side effects
- In-vitro anti-arthritic activity of Abutilon indicum (Linn.) Sweet
- RP-HPLC Method Development and Validation for Estimation of Thiocolchicoside and Diclofenac Potassium in Bulk and Capsule Dosage Forms
- Effects of Vitamin D3 on methotrexate-induced jejunum damage in rats by the utilization of immunohistochemical study
- A review docking studies: In silico methodologies for a better computational approach
- Orally Disintegrating Tablets: A Review
- Insitu Mucoadhesive Nasal Gels of Metoclopramide Hydrochloride : Preformulation and Formulation Studies
- Efficacy of Cynodon dactylon for immunomodulatory activity
- Niosomes: A Review
- Formulation development and evaluation of self-nanoemulsified liquisolid compacts of a poorly soluble biopharmaceutics classification system Class II drug
- Effect of proprioception training and backward walking training among diabetic neuropathy patients
- Antioxidant activity of mangosteen peel (Garcinia mangostana L.) extracted using different solvents at the different times
- Current Trends in Herbal Medicines
- Fascinating diversity and Potent biological activities of Actinomycete metabolites
- Renal abscess due to extended-spectrum beta-lactamase- producing Escherichia coli in a 13-year-old girl