Journal: Drug Invention Today

Article Id: JPRS-P'Col-00005083
Title: Molecular characterization and gene expression profiling of Trichophyton rubrum treated with a Marasmius palmivorus filtrate
Category: Pharmacology
Section: Research Article
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    Background: Trichophyton rubrum is a common anthropophilic fungal species. It is the primary causative dermatophytosis agent in the world, infecting the skin, feet, and the area between the fingernails. Aim: The present study examines the molecular characteristics of T. rubrum isolates and the bioactivity of Marasmius palmivorus filtrate against this fungus. Materials and Methods: Clinical isolates were collected from patients with different varieties of tinea who attended the Al-Hussein Hospital (Dermatology and Venereal Disease Unit) in Kerbala Province from February to November 2018. Results: Four T. rubrum isolates were identified based on their morphological and molecular features and were registered in the International GenBank with the accession numbers: MK167434, MK167437, MK167442, and MK167443, revealing close relationship with National Center for Biotechnology Information-Blast T. rubrum (MH865940.1). The phylogenetic tree of internal transcribed spacer rRNA sequences showed that the T. rubrum isolates (numbers 1, 2, 5, and 6) were closely related to global isolates of the same fungus. The results showed that this fungus could produce protease enzymes. M. palmivorus filtrate (2%, 4%, 6%, and 8%) showed an inhibitory influence on T. rubrum growth through the distortion of fungal structures (presence of abnormal mycelium and cell lysis). Gene expression was detected by quantitative real-time polymerase chain reaction (PCR) using the specific primer β-tubulin (housekeeping gene), a serine protease, citrate synthase, and MEP4. The amplification accuracy of the gene product was observed. The data revealed high gene copy amplification in serine protease, citrate synthase, appeared the accumulation of product through the duration of the qPCR experiment, but on the contrary, it happened with MEP4, which indicates protein lysis. Conclusion: The results of this study will aid in the development of innovative and novel effective drug targets and antifungal agents using M. palmivorus.

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    Author(s) Name:

    Nadia N. H. Al-Masaoodi1, Jawad K. Abood Al-Janabi2*, Ban Taha Mohammed1

    Affiliation(s) Name:

    1 Department of Biology, Pure Science College, University of Kerbala, Iraq,

    2 Department of Pathological Analysis Techniques, AL-Mustaqbal University College, Hilla, Iraq

    *Corresponding author: Jawad K. Abood Al-Janabi, Department of Pathological Analysis Techniques, AL-Mustaqbal University College, Hilla, Iraq.

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    Author:

    Nadia N. H. Al-Masaoodi1, Jawad K. Abood Al-Janabi2*, Ban Taha Mohammed1

    Title:Molecular characterization and gene expression profiling of Trichophyton rubrum treated with a Marasmius palmivorus filtrate
    Journal:Drug Invention Today
    Vol(issue):14 (March)
    Year:2020
    Page No: (109-119)
  • Experimental Methods Keywords

    Methodology:Molecular characterization and gene expression profiling
    Research Materials: Marasmius palmivorus filtrate

Keywords

Marasmius palmivorus filtrate Molecular characteristics Trichophyton rubrum

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