Journal: Drug Invention Today

Article Id: JPRS-P'Col-00005326
Title: Phytochemical analysis and preliminary anti-inflammatory activities of crude Acacia etbaica Schweinf leaf extraction lipopolysaccharide-induced RAW 264.7 macrophage cells
Category: Pharmacology
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    Introduction: Acacia etbaica Schweinf belongs to the family Fabaceae traditionally used to treat stomach pain, swelling, livestock eye infection, itching/scabies, and fire burn. In Ethiopia, the leaves of A. etbaica Schweinf used for the treatment of inflammatory disease. Objective: The present study was conducted to qualitative phytochemical analysis and preliminary in vitro anti-inflammatory activities of A. etbaica Schweinf leaves on lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells. Materials and Methods: Different organic solvent extracts of the dry powdered of A. etbaica Schweinf were obtained by serial exhaustive solvent extraction method using Soxhlet apparatus and was subjected to preliminary phytochemical screening followed by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay for cell viability, Griess assay for nitric oxide (NO) production in LPS-induced RAW 264.7 cells. Results: The phytochemical screening revealed that both acetone and ethanolic leaf extracts of A. etbaica contained significant amount of secondary metabolites such as flavonoids and phenolic compounds. The MTT assay of ethanolic leaf extract of A. etbaica Schweinf at different concentrations (25–500 µg/ml) on RAW 264.7 cells has not shown significant cell toxicity. The NO level was significantly decreased in LPS-induced RAW 264.7 cells at different (25, 50, and 100 µg/ml) concentrations with IC50 of 191.11 ± 1.96 µg/ml. Conclusion: It can be concluded that the ethanolic leaf extract of A. etbaica Schweinf shown significant inhibitory effect on production of NO in LPS-induced RAW 264.7 cells. The preliminary anti-inflammatory activity of the ethanolic leaf extract of A. etbaica Schweinf due to the presence of cardiac glycosides, saponins, flavonoids, tannins, terpenoids, sterols, quinone, anthraquinone, and phenolic compounds.

  • Abstract Audio

    No Audio file found

  • About the authors and Affiliations

    Author(s) Name:

    T. Tekleweyni, H. Kidu, W. Tesfay, K. Krishna Chaithanya, Zenebe Hagos, V. K. Gopalakrishnan*

    Affiliation(s) Name:

    Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia

    *Corresponding author: Dr. V. K. Gopalakrishnan, Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Tigray Region, Post Box No: 1010, Ethiopia.

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    0 View More
  • How to Cite my Article.

    Author:

    T. Tekleweyni, H. Kidu, W. Tesfay, K. Krishna Chaithanya, Zenebe Hagos, V. K. Gopalakrishnan*

    Title:Phytochemical analysis and preliminary anti-inflammatory activities of crude Acacia etbaica Schweinf leaf extraction lipopolysaccharide-induced RAW 264.7 macrophage cells
    Journal:Drug Invention Today
    Vol(issue):14 (February)
    Year:2020
    Page No: (20-25)
  • Experimental Methods Keywords

    Methodology:Anti-inflammatory agents, Cytotoxicity, Nitric oxide, Phytochemical analysis
    Research Materials:Acacia etbaica Schweinf

Keywords

Acacia etbaica Schweinf Anti-inflammatory agents Cytotoxicity Nitric oxide Phytochemical analysis

Our Services

Most Downloaded List