Journal: Drug Invention Today

Article Id: JPRS-P'Col-00005423
Title: Quality of salivary samples collected from porous and non-porous surfaces for dna extraction – A forensic evaluation
Category: Pharmacology
Section: Research Article
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    Introduction: Saliva found on victims can be potentially recovered and typed from bite marks, cigarette butts, postage stamps, envelopes, edibles, and other objects. The detected saliva is recognized as one of the primary source of DNA by the forensic serologist. Aim: In this study, we have evaluated the quality of the salivary samples collected from porous (cloth and mud) and non-porous (cement and ceramic tile) surface for the number of cells obtained and their viability for the use of DNA extraction. Materials and Methods: Sixty samples of saliva were collected on non-porous tile and cement surface and porous cloth surface and saliva was collected at the interval of 2 and 5 days. The samples were collected, centrifuged, and analyzed with trypan blue staining. Results: There was a strong positive correlation between number of cells and viability of cells and the surface, which was statistically significant, rs = 0.726. Conclusion: Saliva is found to be an emerging forensic tool. It has found to be of great help in forensic by monitoring drug abuse, help in sex determination, and bite marks analysis which can be human and non-human. Saliva provides genetic material which can be used even if it is stored suboptimal.

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    Author(s) Name:

    Sinduja Palati*, Pratibha Ramani, Herald. J. Sherlin, S. Gheena, Abilasha Ramasubramanian, K. R. Don, Gifrinajayaraj, Archana Santhanam

    Affiliation(s) Name:

    Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Chennai, Tamil Nadu, India

    *Corresponding author: Sinduja Palati, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, SU, No. 162, Masilamani Nagar, Seneerkuppam Bypass Road, Poonamallee, Chennai, Tamil Nadu, India.

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    Author:

    Sinduja Palati*, Pratibha Ramani, Herald. J. Sherlin, S. Gheena, Abilasha Ramasubramanian, K. R. Don, Gifrinajayaraj, Archana Santhanam

    Title:Quality of salivary samples collected from porous and non-porous surfaces for dna extraction – A forensic evaluation
    Journal:Drug Invention Today
    Vol(issue):13 (March)
    Year:2020
    Page No: (182-184)
  • Experimental Methods Keywords

    Methodology:DNA extraction, Forensics
    Research Materials:Saliva

Keywords

DNA extraction Forensics Non-porous Porous Saliva

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