Journal: Drug Invention Today

Article Id: JPRS-DFP-00005432
Title: Identification of theanine, theobromine, and caffeine from the native Assam variety of Camellia sinensis (green tea)
Category: Drugs from Plants
Section: Research Article
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    Camellia sinensis (green tea) is commonly consumed as beverage, which has several health benefits. It is widely grown in various climatic regions and its chemical composition is quite complex, but varies based on its geographical conditions seasonal variations and other environmental factors. It consists of numerous bioactive compounds, such as polyphenols, amino acids, and methylxanthines. Still, many more has to be explored. Moreover, none of the studies from India reported the presence of theanine, theobromine, and caffeine in native (Assam) variety of green tea which has clinical importance. Thus, the present study is conducted to assess the above-mentioned molecules in native (Assam) green tea. Green tea leaves from Assam were used to prepare aqueous and ethanolic extracts by Soxhlet apparatus. Later, we compared and analyzed the chemical composition of these extracts by liquid chromatography and mass spectrometry spectral analysis. We assessed the chemical composition between the leaf extracts and identified an important amino acid theanine (C7 H14 N2 O3, mass 174.2 m/z), and methylxanthines like theobromine (C7 H8 N4 O2, mass 180.16 m/z), and caffeine (C8 H10 N4 O2 , mass 194.19 m/z) in both the extracts. Thus, we conclude the presence of theobromine, theanine, and caffeine in both the leaf extracts. Moreover, the present study also suggests that minimal intake of green tea has beneficial effects, whereas maximum intake has side effects.

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    Author(s) Name:

    Sarvepalli Vijay Kumar1, Amiya Bhaumik2, Krishnan Ramalingam3, Pasupuleti Sreenivasa Rao4,5,6 *, Perali Ramu Sridhar7

    Affiliation(s) Name:

    1 Department of Paediatrics, Lincoln University College, Kuala Lumpur, Malaysia,

    2 Lincoln University College, Kuala Lumpur, Malaysia,

    3 Department of Biochemistry, Narayana Medical College and Hospital, Nellore, Andhra Pradesh, India,

    4 Department of Biochemistry, 5 Central Research Laboratory ( Dept of ARC ) , Narayana Medical College and Hospital, Nellore, Andhra Pradesh, India,

    6 Department of Pharmacology, Narayana College of Pharmacy, Nellore, Andhra Pradesh, India,

    7 School of Chemistry, University of Hyderabad, Gachibowli, Hyderabad, India

    *Corresponding author: Dr. Pasupuleti Sreenivasa Rao, Department of Biochemistry, Central Research Laboratory( Dept of ARC ), Narayana Medical College and Hospital, Nellore - 524 003, Andhra Pradesh, India.

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    Author:

    Sarvepalli Vijay Kumar1, Amiya Bhaumik2, Krishnan Ramalingam3, Pasupuleti Sreenivasa Rao4,5,6 *, Perali Ramu Sridhar7

    Title:Identification of theanine, theobromine, and caffeine from the native Assam variety of Camellia sinensis (green tea)
    Journal:Drug Invention Today
    Vol(issue):14 (March)
    Year:2020
    Page No: (63-71)
  • Experimental Methods Keywords

    Methodology:Identification , Preparation of Extracts,LC–mass Spectral Analysis
    Research Materials: Camellia sinensis

Keywords

Camellia sinensis Secondary metabolites Traditional medicine

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