Journal: Drug Invention Today

Article Id: JPRS-P'Col-00005438
Title: Economic tissue microarray using stamp and punch biopsy needle
Category: Pharmacology
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    Introduction: Conventionally, paraffin blocks were utilized to diagnose the histological samples. Tissue microarrays make the analysis of these histological samples easy by compiling all the information on a single slide. A paraffin block with an array is prepared, and a needle transfers samples from the histological sample blocks into it. Different tissue samples from various tissue blocks are punched and re-embedded on a new paraffin block. Aim: The aim of this study is to fabricate an economic tissue microarray using stamp and punch biopsy needle. Materials and Methods: The stamp was fabricated with 2 mm cylindrical template arranged in three rows and three columns. The molten paraffin wax was poured into the leukardt mold. The stamp with the cylindrical template was placed over it when the wax was in the molten state itself after which the wax was allowed to set. After that, the stamp with a cylindrical template was retrieved. This creates numerous holes in the wax block. Punch biopsy needle 2 mm diameter with the plunger was used to punch the tissue from the previous paraffin- embedded tissues. The punched tissue from various paraffin blocks was transferred into the holes created by a stamp in the paraffin block. A hot plate is used to stabilize it after placing all the punches of tissue. The paraffin block with arrayed tissues was sliced using microtomy and transferred to the slide and viewed under microscope or can be used for molecular and immunohistochemistry procedures. Results: In this study, tissue microarray procedure was done using a stamp and punch biopsy needle which is economical and accurate. Nine tissues were transferred to slides, and both epithelium and connective tissue were seen clearly without any fold and tear.

  • Abstract Audio

    No Audio file found

  • About the authors and Affiliations

    Author(s) Name:

    K. Hema Shree*, Pratibha Ramani, S. Gheena, Abilasha Ramasubramanian, Monika Karunagaran, R. Hannah

    Affiliation(s) Name:

    Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: K. Hema Shree, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    0 View More
  • How to Cite my Article.

    Author:

    K. Hema Shree*, Pratibha Ramani, S. Gheena, Abilasha Ramasubramanian, Monika Karunagaran, R. Hannah

    Title:Economic tissue microarray using stamp and punch biopsy needle
    Journal:Drug Invention Today
    Vol(issue):14 (March)
    Year:2020
    Page No: (93-95)
  • Experimental Methods Keywords

    Methodology: Punch biopsy needle, Economic tissue microarray
    Research Materials:tissue

Keywords

Economic tissue microarray Punch biopsy needle Stamp

Our Services

Most Downloaded List