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Abstract

Tannins are complex moieties produced by majority of plants as protective substances having wide pharmacological activities. They have been used since past as tanning agents and reported for astringent, anti-inflammatory, antidiarrhoeal antioxidant and antimicrobial activities. Because of their chemical complexity and difficulty in isolation due to their close association with other compounds, it is necessary to estimate the total tannins. In the present work we have tried to estimate the total tannins in different parts of the plant by simple spectrophotometric method. Gallic acid was used to obtain standard curve and 30min refluxed extracts of all parts with suitable dilutions (beer limit 10-22 µg) were analyzed at 700nm. Calibration curve shows coefficient of variance (r2) = 0.9994 and tannin content was found to be highest in bark (2.4125 ± 0.124) then in leaves (1.7586 ± 0.18), roots (1.3318 ± 0.16) and Stem bark (1.0538 ± 0.16), all collected in the month of Feb-March. Inflorescence showed minimum amount of tannins (0.08639 ± 0.064). Also estimations were made repeatedly with fresh and stored samples (up to 3 years) and found no significant difference in tannin content. Maximum average variation was found in bark (0.0037%) and minimum (0.0011%) in stem wood compare to fresh sample. The method was found good and reproducible. This study revels that all the parts tested contains tannin and no significant variation was found in fresh and stored samples indicates tannins are stable up to three years if stored properly.

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	JPR Solutions

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