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Abstract

In the present investigation antimicrobial activity of two Indian medicinal plants i.e. Tinospora cordifolia and Cassia fistula were evaluated against seven human pathogenic bacterial strains. Both were tested by serial micro-dilution method. Antimicrobial susceptibility of aqueous and solvent extracts was assessed. For this purpose, both positive and negative controls were set to determine MIC and MBC values. After bioassays, aqueous and solvent extracts of Tinospora cordifolia and Cassia fistula exhibited significant antimicrobial susceptibility against bacteria both Gram negative and Gram positive i.e. Klebsiella pneumoniae (ATCC 15380), Escherichia coli (ATCC 25922), Micrococcus luteus (ATCC 9341), Streptococcus pneumoniae (ATCC 12755), Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 11778) and Lactobacilus acidophilus (ATCC 53103) at a very low concentration. More specifically, higher percent growth inhibition was obtained in presence of aqueous extracts in comparison to solvent extracts, which was much higher than synthetic antibiotics. Further, different extracts have shown very low MIC value, which was obtained in a range of 0.0078-0.125 mg/ml. It was much lower than the MIC values (0.223-0.892 mg/ml) obtained in presence of standard antibiotics i.e. tetracycline, ampicillin and ciprofloxacin. More specifically, aqueous extracts of both plants have shown lower MIC (0.00975 mg/ml in E. coli and K. pneumoniae) and MBC values (0.078 mg/ml in E. coli and K. pneumoniae) than broad-spectrum antibiotics (0.223-0.892 mg/ml). Further, aqueous extracts of both plant species have shown significantly much higher inhibition zone diameters (20-30mm) against all seven bacterial strains than the synthetic antibiotics (6-18). Certainly, above antimicrobial effects are attributed due to presence of certain chemical substances in the leaves and legumes of above plant species.

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	JPR Solutions

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